Data First. Science Driven.
Cytorum Services
Always putting data first, we specialize in complex assays and challenging research and clinical projects.
Technology
Single Cell Analysis Platforms
Experts in both fluorescence and mass cytometry, we can help you select the best platform for your studies. Whether you want to analyze a few targets or dozens of targets per cell, we can design, optimize, and build complex staining panels.
Measuring biological responses by flow cytometry presents enormous opportunity to evaluate compound activity and cellular responses across different cell types in highly relevant human primary cells. Utilizing whole blood, purified PBMCs, or diseased samples, these mixed populations can be interrogated in detail for their responses to small molecules, antibody therapeutics, cytokines, growth factors, and antigen stimulation. Whether the subject is patient samples or medium throughput compound characterization, the ability to monitor multiple response markers across a range of cell subsets makes flow cytometry an ideal solution. Cytorum can help you design the most efficient and robust path forward in implementing flow cytometry across a range of applications.
Measuring biological responses by flow cytometry presents enormous opportunity to evaluate compound activity and cellular responses across different cell types in highly relevant human primary cells. Utilizing whole blood, purified PBMCs, or diseased samples, these mixed populations can be interrogated in detail for their responses to small molecules, antibody therapeutics, cytokines, growth factors, and antigen stimulation. Whether the subject is patient samples or medium throughput compound characterization, the ability to monitor multiple response markers across a range of cell subsets makes flow cytometry an ideal solution. Cytorum can help you design the most efficient and robust path forward in implementing flow cytometry across a range of applications.
Fluorescence Flow Cytometry
The gold standard of single cell analysis, fluorescence flow cytometry is the most widely used platform for immunological analysis. Take advantage of our expertise in solving the most complex flow assays and optimizing for signal-to-noise, reproducibility and quantitation.
Fluorescence panels: 10-18 parameters
Acquire millions of cell for rare subsets or large sample sets
Leverage published staining panels or design your own
Identify the best antibody clones and fluorophores for your targets
Custom conjugate unique reagents or therapeutic antibodies
Mass Cytometry
With its ability to analyze over 40 parameters per single cell, mass cytometry (CyTOF) can streamline sample analysis by combining 3 or 4 staining panels into one. Due to minimal overlap between channels, mass cytometry enables more rapid development of 40 parameter staining panels than spectral cytometry.
CyTOF panels: ~40 parameters including barcoding, viability
Our experience conjugating antibodies for CyTOF lets you design the optimal staining panel
Surface and intracellular targets (cytokines, phospho-proteins, histones)
ViSNE, t-SNE data visualization
Robotic sample processing for optimal cell recovery
Spectral Fluorescence Cytometry
The next generation of fluorescence flow cytometry, spectral analyzers measure 50 or more channels per cell, enabling novel combinations of new fluorophores to be used.
Spectral panels of 25-35 markers
Dive deeply into specific or rare cell subsets
Get all the information you can from a single panel
Combine new dyes with gold standard dyes for maximum signal to noise
Unprecedented insight into cell function and phenotype
Data Analysis
We understand the nuances of flow cytometry and single cell data. Leverage our expertise to turn your complex multi-parameter experiments into quantifiable and actionable data sets.
Optimized assays ensure optimal resolution and reproducibility. Good experiments generate good data.
Evaluate data in ways that are appropriate for the questions being asked: means, medians, and percent positive
We routinely handle large flow cytometry data sets to define the most important metrics and uncover correlations that might be missed
Gating: optimize gating strategies to identify key cell subsets
Visualize CyTOF data with dimensionality reduction, t-SNE, viSNE, etc
Solutions
Application Areas
Experts in both fluorescence and mass cytometry, we can help you select the best platform for your studies. Whether you want to analyze a few targets or dozens of targets per cell, we can design, optimize, and build complex staining panels.
Biomarker Assays
Develop biomarker assays to measure pharmacodynamics or on-target and off-target activity in whole blood and PBMCs
Receptor Occupancy (PD-1, CD38, CTLA-4, etc)
Phospho-proteins (ERK, Akt, Jak-Stat, etc)
Epigenetic markers (methylation, acetylation of histones)
Surface or intracellular protein expression or degradation
Cell cycle and proliferation
Therapeutic Development
Assess therapeutic activity in whole blood. Rank compounds using multiparameter analysis on primary human cells.
Dose response analysis
Medium-throughput compound screening
Evaluate multiple markers of drug activity simultaneously
Test compounds on healthy donor or disease samples
Process blood and tissue samples from animal studies
Projects
Custom Projects: Design, Execute and Analyze
Let Cytorum help you design the optimal set of experiments, then perform those experiments in our laboratory. Mix and Match inputs, sample types and readouts to create the exact experiment you need.
Inputs
Add both small molecules and antibody therapeutics to induce or inhibit cell functions. Activate cells with cell-type specific crosslinking or general cytokine stimulations. Perform dose response, time course experiments.
Small molecule drugs
Antibody therapeutics
Cell activation (T/B cell crosslinking)
Cytokine stimulation
Dose response, time course
Sample Types
Human whole blood and PBMCs provide the most relevant sample types for human immunology and IO studies. Perform pre-clinical analysis in mouse and other species.
Human whole blood and PBMCs
Normal healthy donors, patient samples
Human cell lines
Bone marrow and complex tissues
Animal samples (mouse, rat, cyno)
Readouts
If you can measure it by flow cytometry, we have probably done it. We specialize in intracellular staining for difficult targets for drug screening, or customized readouts of cell and drug activity for biomarker studies.
Cell surface (CD) markers
Phospho-proteins (signaling)
Intracellular proteins (cytokines)
Cellular differentiation and activation
"I want to do what is in this paper I found"
How it works
Project Design to Data Delivery
Target selection
Select the markers you want to analyze. Measure cell surface or intracellular proteins, or the levels of your protein therapeutic bound to cells.
Technology selection
Fluorescence or mass cytometry? Imaging? We can help select the best platform for your markers.
Design the panel
Optimize for your most important markers of interest to enhance signal-to-noise and reproducibility.
Analyzing Data
We use several tools across our informatics pipeline, from raw data analysis in CellEngine to data visualization in Spotfire.
Deliver Results
We deliver data in more meaningful ways, helping you interpret the results, and reducing the complexity of high parameter cell analysis.