Data First. Science Driven.
Advancing Single Cell Analysis
Always putting data first, we provide products and services that enable next generation cellular analysis
Technology
Single Cell Analysis Platforms
Experts in both fluorescence and mass cytometry, we can help you select the best platform for your studies. Whether you want to analyze a few targets or dozens of targets per cell, we can design, optimize, and build complex staining panels.
Fluorescence Cytometry
The ideal solution for analyzing ~10-15 markers per cell across large sample sets for drug discovery, preclinical animal studies, and human clinical studies.
Spectral Cytometry
High parameter fluorescence cytometry analyzing 25+ markers per cell. Ideal for biomarker discovery, deep immunophenotyping and rare cell analysis.
Mass Cytometry
When you need even more markers, mass cytometry enables analysis of ~40+ markers per cell for biomarker discovery and deep analysis of precious samples.
Assays and Biomarkers
We specialize in complex assay systems:
Intracellular protein analysis: total proteins, transcription factors
Phospho-proteins and cell signaling
Receptor occupancy for antibody therapeutics
Biomarker discovery: cell surface and intracellular targets
Ex vivo stimulation of blood and PBMC samples with cytokines/ligands
Immunophenotyping
Tailored solutions for your target and sample type
Fluorescence panels: 18 colors across five lasers
Spectral and CyTOF panels: 25-40 parameters including barcoding, viability
Custom antibody conjugation for both mass and fluorescence
Surface and intracellular targets (cytokines, phospho-proteins, histones)
Human, mouse, rat, canine; analyze preclinical to clinical samples
Projects
Custom Projects: Design, Execute and Analyze
Let Cytorum help you design the optimal set of experiments, then perform those experiments in our laboratory. Mix and Match inputs, sample types and readouts to create the exact experiment you need.
Inputs
Add both small molecules and antibody therapeutics to induce or inhibit cell functions. Activate cells with cell-type specific crosslinking or general cytokine stimulations. Perform dose response, time course experiments.
Small molecule drugs
Antibody therapeutics
Cell activation (T/B cell crosslinking)
Cytokine stimulation
Dose response, time course
Sample Types
Human whole blood and PBMCs provide the most relevant sample types for human immunology and IO studies. Perform pre-clinical analysis in mouse and other species.
Human whole blood and PBMCs
Normal healthy donors, patient samples
Human cell lines
Bone marrow and complex tissues
Animal samples (mouse, rat, cyno)
Readouts
If you can measure it by flow cytometry, we have probably done it. We specialize in intracellular staining for difficult targets for drug screening, or customized readouts of cell and drug activity for biomarker studies.
Cell surface (CD) markers
Phospho-proteins (signaling)
Intracellular proteins (cytokines)
Cellular differentiation and activation
"I want to do what is in this paper I found"
How it works
Project Design to Data Delivery
Target selection
Select the markers you want to analyze. Measure cell surface or intracellular proteins, or the levels of your protein therapeutic bound to cells.
Technology selection
Fluorescence or mass cytometry? We can help select the best platform for your markers.
Design the panel
Optimize for your most important markers of interest to enhance signal-to-noise and reproducibility.
Analyzing Data
We use several tools across our informatics pipeline, from raw data analysis in CellEngine to data visualization in Spotfire.
Deliver Results
We deliver data in more meaningful ways, helping you interpret the results, and reducing the complexity of high parameter cell analysis.